Dyeing mechanism of uranium dioxy acetate
As a commonly used dye, uranium dioxy acetate has a delicate and complex dyeing mechanism. This dyeing process involves many chemical and physical effects.

From a chemical point of view, uranium dioxy acetate will ionize in solution, resulting in positively charged uranium ions and their related ionic groups. These cations can attract certain negatively charged groups in biological samples or other substances to be dyed. Biological macromolecules such as proteins and nucleic acids, because their structures contain acidic groups such as phosphate groups and carboxyl groups, can ionize negative charges under appropriate conditions. Uranium ions combine with these negatively charged groups through electrostatic attractive forces, thus realizing the initial binding and localization of the sample.

From a physical perspective, the heavy metal composition of uranium acetate can increase the scattering ability of electrons in different parts of the sample after staining. When observed by electron microscope, the stained area forms a significant contrast difference with the surrounding area due to high electron scattering. In this way, the fine structure of the sample can be clearly presented. This combination of physical effect and chemistry works together to improve the staining effect, so that the structural details of the sample can be clearly displayed, enabling researchers to further study the microstructure and morphological characteristics.