China Triacetin, Acetate Series Suppliers, Manufacturer

Tris Acetate Running Buffer Recipe

Tris Acetate Electrophoresis Buffer Formula
1. Required Materials
1. ** Tris Base **: Analytical purity level, used to provide the basic component of the buffer system.
2. ** Glacial Acetic Acid **: Superior purity, used as an acidic component to co-regulate the pH value of the buffer with Tris Base.
3. ** Disodium Ethylenediamine Tetraacetic Acid (EDTA - Na ²) **: Analytical purity, the main function is to chelate the metal ions in the solution to prevent its possible interference with the experimental process.
4. ** Deionized Water **: To ensure the purity of the solution and avoid the influence of impurities on the experiment.

2. Preparation steps
1. ** Prepare a suitable container **: Select a clean and suitable volume glass beaker or plastic container for preparing the buffer.
2. ** Weigh Tris base **: Weigh a certain quality of Tris base accurately according to the formula and put it in the prepared container. For example, if 1L of Tris acetate electrophoresis buffer with a concentration of 0.05mol/L is to be prepared, according to the molar mass of Tris base (121.14g/mol), it is calculated that the mass of Tris base should be weighed as\ (0.05mol/L × 1L × 121.14g/mol = 6.057g\).
3. ** Add glacial acetic acid **: Use a pipette to accurately measure an appropriate amount of glacial acetic acid and add it to the container. Take the preparation of the above 1L buffer as an example, assuming that the volume of glacial acetic acid required is\ (V\) mL, according to the final concentration of acetic acid in the buffer and the density (1.05g/mL) and purity (generally 99.5% - 100%) of glacial acetic acid, determine the\ (V\) value through relevant calculations and measure and add it.
4. ** Add EDTA - Na 2O **: Accurately weigh an appropriate amount of EDTA - Na 2O and add it to the container. For example, to make the concentration of EDTA-Na ³ in the buffer reach 0.001mol/L, based on its molar mass (372.24g/mol), it is calculated that the weight should be\ (0.001mol/L × 1L × 372.24g/mol = 0.37224g\).
5. ** Add deionized water to dissolve and bandwidth evaluation **: Add an appropriate amount of deionized water to the container, stir to fully dissolve the ingredients, and after complete dissolution, transfer the solution to a volumetric flask and use deionized water bandwidth evaluation to the desired volume, as in the above example bandwidth evaluation to 1L.
6. ** Adjust the pH value **: Use a pH meter to measure the pH value of the solution, and fine-tune it with glacial acetic acid or Tris alkali solution as needed to make the buffer reach the required pH value. Generally, the pH value of Tris acetate electrophoresis buffer is around 8.0 - 8.5.

III. Precautions
1. ** Material quality **: Ensure that the quality of the materials used such as Tris alkali, glacial acetic acid, EDTA-Na ³ is qualified, and avoid affecting the performance of the buffer due to impurities.
2. ** Operating specifications **: During the operation process of weighing and measuring, strictly follow the experimental specifications to ensure the accuracy of measuring.
3. ** pH adjustment **: The pH value should be adjusted slowly to avoid excessive adjustment. After each adjustment, wait for the pH meter reading to stabilize before proceeding to the next step.
4. ** Storage conditions **: The prepared buffer should be stored in a clean container, usually in a refrigerator at 4 ° C, to avoid microbial contamination and changes in buffer composition.