On the gist of Tris-Acetate-Edta Buffer

Taste of things, the name is Tris-Acetate-Edta Buffer, its nature is different, and it is not fine for various uses. This thing is also in the field of biochemistry, and there are many actions.

View its properties, it has a certain stability, can be in a specific environment, and maintains its characteristics without changing. In the solution, it can be a medium, so that all reactions can proceed in sequence. And the ratio of its components is exquisite. Tris can adjust the pH of the solution, so that it can reach a suitable environment, so as to facilitate all kinds of biochemical activities. Acetate is also useful, which helps to form a buffer and cooperates with Tris to make the acid-base balance stable and not shaken. And Edta is in it, which can chelate metal ions to prevent them from disturbing during the reaction and damaging the process.

As for the preparation method, there are also rules. All kinds of raw materials must be taken with accurate measurement. When dissolved, it needs to be advanced in sequence, and stirred to ensure that it is uniform. Also pay attention to changes in temperature, too high or too low, can cause its quality to be different. After preparation, it is still necessary to check its purity and measure its properties to ensure that it meets the standards used.

It is also widely used. In the analysis of nucleic acids, it is often necessary. In the experiment of electrophoresis, it is relied on as a buffer, so that the nucleic acid molecules can swim in an orderly manner in the glue according to the charge and size, so as to form a clear strip, so that researchers can analyze its structure and determine its sequence. In the cloning of genes, it is also indispensable. It can maintain the stability of the reaction system, make the activity of enzymes clear, and make the splicing and replication of gene fragments smooth.

Use this product with caution. Because of its ingredients, or irritating, if you accidentally touch the skin, rinse it with water quickly, and seek medical treatment for those who are serious. When storing, it should be placed in a cool and dry place, protected from heat and light, to prevent its deterioration.

In general, Tris-Acetate-Edta Buffer has made great contributions to biochemistry. Those who use it, know its nature, understand its methods, and act carefully, will be able to use its power to promote the research of growth and transformation, and get fruitful results.