On the method of Tris-acetic acid-ethylenediaminetetraacetic acid buffer electrophoresis
Now there is a method of Tris-acetic acid-ethylenediaminetraacetic acid buffer electrophoresis, which is very important in the field of biochemistry. The method uses Tris, acetic acid, and ethylenediaminetraacetic acid to mix in an appropriate ratio to make the buffer solution.
This buffer electrophoresis can be divided into steps. Prepare the utensils first, clean and stain-free, to ensure the accuracy of the experiment. For secondary buffer solution, measure Tris, acetic acid, and ethylenediaminetraacetic acid, dissolve them in pure water according to a specific example, stir them well, measure their pH, and adjust them to an appropriate value to meet the needs of electrophoresis.
Then the sample is taken and added to the gel after appropriate reasons. The gel is the basis of electrophoresis, and the preparation also needs to be cautious. According to the nature of the sample, choose a suitable gel, control the temperature and control the time, and ensure that the gel is homogeneous.
And the post-gel is placed in the electrophoresis tank, fill the buffer solution, and stop flooding the gel. Connect the power supply and set the appropriate voltage and current to make the sample swim in the gel. Observe the migration of the sample and stop it in time.
Tris-acetic acid-ethylenediaminetetraacetic acid buffer electrophoresis can analyze the genus of nucleic acid and protein. It can be divided into objects of different sizes and charges, and is very useful in scientific research and medical treatment. It is also a tool for understanding the microscopic world.