On the preparation of a certain reagent and the use of
existing reagent, the name is Tris + Acetate + Edta Buffer 50x, which is widely used and has great functions in various experiments.

The preparation of this agent should also be done in a rigorous way. First take an appropriate amount of Tris, which is the basis of the agent. Its properties are mild, and it is effective in adjusting the acid-base balance of the solution. Then measure Acetate, according to a certain ratio, and mix it with it. Acetate has a specific chemistry, which is coordinated with Tris, making the agent more stable. Added Edta, which can chelate metal ions, protect the quality of the agent, and avoid the disturbance of impurities.

When all things are polymerized, mix them with water, stir well, and make sure they are fully integrated. And in the process of preparation, you need to pay attention to temperature, pressure, etc., subtle differences, or changes in the properties of the agent.

As for the use, it should be diluted first. With it as a concentrated agent of 50x, it must be diluted with water according to the needs of the experiment. In the genus of electrophoresis, this agent can stabilize the electric field environment, so that the molecular migration is orderly and the bands are clear. In other biochemical experiments, it can also adjust the state of the solution and help the reaction go smoothly.

Make good use of this Tris + Acetate + Edta Buffer 50x, then the experimental power can be expected to be successful. The experimenter should be careful with the system and be good at using it in order to achieve the best results.