Preparation of triacetic acid buffer
Now we want to make triacetic acid buffer, which is often used in biochemical experiments. The preparation method requires precise steps and dosage to obtain a suitable buffer.

Prepare the required drugs and utensils first. Drugs include trimethylolaminomethane (Tris), glacial acetic acid, etc. The equipment must be balanced, volumetric bottles, glass rods, pH meters, etc., to ensure accurate operation.

Weigh an appropriate amount of Tris in a clean beaker, and the dosage depends on the concentration and volume of the required buffer. If you want to make a specific concentration and volume of buffer, calculate and weigh a certain quality of Tris. Dissolve it with an appropriate amount of distilled water. During the dissolution process, gently stir with a glass rod to help it dissolve quickly.

Then, slowly add glacial acetic acid. When adding acid, pay close attention to the pH meter, because the amount of glacial acetic acid is related to the pH value of the buffer. Add dropwise until the pH meter reaches the desired value. This process requires patience and meticulousness to prevent excessive deviation of the pH value.

When the pH value is adjusted to a suitable level, transfer the solution to the corresponding volume of volumetric flask. When transferring, drain the water with a glass rod to ensure that the solution does not spill. Then rinse the beaker and glass rod with a small amount of distilled water several times, and the lotion is also incorporated into the volumetric flask to avoid solute loss.

Finally, add distilled water to the scale line of the volumetric flask, and the bandwidth evaluation is completed. Cover the stopper and turn the volumetric flask upside down several times to fully mix the solution. At this point, the triacetic acid buffer is prepared and can be used for subsequent experiments.