The method of trimethylol-aminomethane-acetic acid buffer calculator
If you want to make trimethylol-aminomethane-acetic acid buffer, when you know the method. The first thing to know is the properties of trimethylol-aminomethane (Tris), which is an organic base and is often used as a buffer in biochemical experiments.

To use this calculator, first determine the pH of the desired buffer. Due to different experimental requirements, pH varies. If it is protein electrophoresis, a buffer with a pH of about 8.3 is often required.

Then, determine the concentration of Tris. Its concentration is related to the buffer capacity of the buffer. General biochemical experiments, the commonly used concentration is between 0.05M - 0.5M. High concentration has strong buffering capacity, which may affect the experimental system.

Furthermore, calculate the amount of acetic acid. Acetic acid reacts with Tris to form a buffer pair. According to the required pH and Tris concentration, the amount of acetic acid can be obtained through a specific formula. This formula is based on the acid-base balance theory and is accurately calculated.

Also, when dosing, pure water should be used. The purity of water is related to the purity of the buffer, so as not to affect the experiment. After dosing, check it with pH. If the pH does not match, fine-tune the amount of acetic acid or Tris until it is desirable.

In this way, according to the method of trimethylol-aminomethane-acetic acid buffer calculator, a suitable buffer can be prepared for biochemical experiments.