The method of potassium acetate DNA precipitation
Now there is a method of extracting and purifying DNA, and potassium acetate precipitation is quite important.

To precipitate potassium acetate DNA, first take an appropriate amount of the solution containing DNA. This solution may come from the cleavage of biological tissue or come from elsewhere. Then, add an appropriate amount of potassium acetate solution. Potassium acetate, in the solution, can interact with many impurities to change its properties.

When potassium acetate is integrated into a DNA-containing solution, potassium ions and acetate ions play a role. Potassium ions can combine with negatively charged impurities in the solution, and acetate ions also participate in the reaction, resulting in a change in the solubility of the impurities, which in turn forms a precipitation. At this time, DNA, due to its special structure and properties, still exists in the solution.

Subsequently, the mixed solution is treated by centrifugation. During centrifugation, under the action of strong centrifugal force, the formed precipitate settles at the bottom of the tube, while the supernatant containing DNA remains in the upper layer. Carefully absorb the supernatant to obtain a preliminary purified DNA solution.

This method of potassium acetate DNA precipitation has a significant effect on DNA extraction and purification. It can effectively remove impurities and improve DNA purity, laying a good foundation for subsequent molecular biology experiments such as gene sequencing and PCR amplification.