On the Method of Potassium Acetate-Acetic Acid Buffer
There is a thing today, the name is potassium acetate-acetic acid buffer, which has extraordinary functions in all kinds of biochemical things. This liquid can stabilize the acid and base of the system, protect the properties of the substance, and make everything go smoothly.
It is also necessary to make this buffer in advance. For acetic acid, weak acid is also; for potassium acetate, its salt is also. The two are compatible. According to the law of chemical equilibrium, they can resist the disturbance of external acid and base. To obtain a suitable buffer, you need to measure the ratio of the two.
Take an appropriate amount of potassium acetate and dissolve it in water. The water should be pure and free of impurities and filth. Then slowly add acetic acid, and use a precise acid measuring instrument to observe the change of its pH. Until its value is suitable for the required environment, it will stop. Among them, if there is more potassium acetate, it will have strong anti-acid power; if acetic acid is rich, it will have anti-alkali power.
When using this buffer, you should also be cautious. Biochemical experiments are delicate, and when encountering unsuitable acid and alkali, they will change and fail. Protecting it with potassium acetate-acetic acid buffer can ensure the stability of the product, and its response will be smooth. In the test of enzymes, the analysis of proteins and other things, all rely on this liquid.
It is said that potassium acetate-acetic acid buffer is an indispensable thing in the process of biochemistry. Only by making good use of it can we make everything smooth, explore the subtleties, and obtain the true meaning of science.