On the method of testing vitamin E acetate
To test vitamin E acetate, there are three methods.

First, you can borrow the method of high-performance liquid chromatography. Prepare an appropriate amount of the sample, flow and dissolve it, and inject it into the liquid chromatograph. Adjust the appropriate flow rate, column temperature and other parameters, until it is balanced. Looking at the chromatogram, vitamin E acetate has its specific retention time, which can be determined by its authenticity compared with the retention time of the standard product. And by the peak area, according to the external standard method or the internal standard method, its content can be measured. This is an accurate method, but it needs to be well-equipped and the operator is familiar with the instrument.

Second, use the method of gas chromatography. The sample is first treated with derivatization to increase its volatility. The gasification chamber is set up to gasify, and the carrier gas is entered into the chromatographic column. According to the difference in its distribution coefficient in the column, it flows out one after another and is detected by the detector. The peak shape and retention time of vitamin E acetate are the criteria, and its quality and quantity can be known. This method has high separation efficiency, fast analysis speed, and is quite good for the detection of volatile substances.

Third, the method of chemical identification. Vitamin E acetate has the characteristics of esters. Add alkali hydrolysis to obtain vitamin E and corresponding salts. Vitamin E is reductive and can react with ferric chloride test solution to show a specific color to prove the existence of vitamin E acetate. Although this method is convenient, it is slightly less specific, or it needs to be supplemented by other methods.

The test of vitamin E acetate should be selected according to the situation, or the number method should be used to obtain confirmation.