Method for making triacetic acid gel
To make triacetic acid gel, first prepare all the ingredients. It is necessary to have trimethylaminomethane (Tris), glacial acetic acid, ethylenediaminetraacetic acid (EDTA), and acrylamide, methylenebisacrylamide and other reagents. In addition, polymerization catalysts such as ammonium persulfate and accelerator tetramethylethylenediamine are also indispensable.

Make a buffer first. Add Tris in an appropriate amount of pure water in an appropriate proportion, stir slowly, and wait for it to dissolve fully. Then, add glacial acetic acid dropwise, and adjust it to a suitable pH as measured by pH. Add a certain amount of EDTA, stir well, and the buffer is formed.

Then prepare a gel solution. According to the gel concentration required by the experiment, accurately weigh acrylamide and methylenebisacrylamide and dissolve in the above-mentioned prepared buffer solution. The dissolution process needs to be stirred patiently, or moderately heated to promote its dissolution, but the temperature should not be too high, so as not to affect the properties of the reagent.

After the gel solution is uniform, add an appropriate amount of ammonium persulfate and tetramethylethylenediamine. After these two are added, the gel starts to polymerize, so the action should be rapid. The mixture is carefully injected into the prepared gel mold, and a certain space is reserved for insertion into the comb to form a sample hole.

After injection, let it stand in a suitable environment until it is fully polymerized. The polymerization time varies depending on the temperature and the amount of reagent, usually taking tens of minutes to several hours. When the gel is completely polymerized, carefully remove the comb. At this time, the triacetic acid gel is prepared and can be used for subsequent experimental analysis.