Method for making tris (hydroxymethyl) aminomethane acetic acid buffer

If you want to make tris (hydroxymethyl) aminomethane acetic acid buffer, you must specify the steps and abide by the procedures.

First, prepare the required materials and utensils. For materials, tris (hydroxymethyl) aminomethane, glacial acetic acid, and ultrapure water are also available; for utensils, the balance should be weighed by weight, the measuring cylinder should be used to measure the body of the liquid, the volumetric bottle should be used to measure the amount of the liquid, the stirring rod should be used to promote the melting of the substance, and the pH meter should be used to measure the acid and alkali of the liquid.

Times, weigh an appropriate amount of tris (hydroxymethyl) aminomethane and put it in a clean beaker. According to the concentration and volume of the required buffer, after accurate calculation, the required mass of tris (hydroxymethyl) aminomethane is obtained, and it is carefully weighed in a balance without deviation.

Next, measure an appropriate amount of glacial acetic acid. Glacial acetic acid is corrosive and should be handled with caution. Use a measuring cylinder to measure according to the calculated amount, and slowly pour it into a beaker containing tris (hydroxymethyl) aminomethane.

Then, add ultrapure water. Add some ultrapure water first, and stir slowly with a stirring rod to promote the fusion of tris (hydroxymethyl) aminomethane with glacial acetic acid. When stirring, use force to stabilize and do not splash the liquid out.

After initial melting, remove this liquid to a volumetric flask. Rinse the beaker and stirring rod several times with a small amount of ultrapure water, wash the liquid and move it to the volumetric flask to ensure that the solute is intact.

Add ultrapure water to the vicinity of the scale line of the volumetric flask, and use a dropper to add it dropwise until the concave part of the liquid level is tangent to the scale line.

Finally, measure the pH value with pH. If the pH value is not required, add glacial acetic acid or tris (hydroxymethyl) aminomethane solution dropwise to fine-tune until a suitable pH value is obtained. After adjusting, close the volume cork tightly and shake it upside down repeatedly to make the buffer uniform.

In this way, tris (hydroxymethyl) aminomethane acetic acid buffer is prepared. During the operation, be sure to focus carefully to obtain a suitable buffer.