How To Make Ammonium Acetate Buffer
Method for making ammonium acetate buffer
To make ammonium acetate buffer, follow the following method. First, you need to prepare the materials used, acetic acid ($CH_3COOH $) and ammonia ($NH_3\ cdot H_2O $) are necessary, and accurate measuring instruments are required, such as measuring cylinders, balances, etc.

First, according to the concentration and volume of the required buffer, calculate the amount of acetic acid and ammonia. If you want to make a buffer with a specific pH value, according to the calculation formula of the buffer solution pH value $pH = pKa +\ lg\ frac {[conjugated base]} {[conjugated acid]} $ ($pKa $of acetic acid is a constant, about 4.76 dollars $), deduce the appropriate ratio of acetic acid to ammonia water. When measuring

, accurately measure the required volume of acetic acid in a measuring cylinder and pour it into a clean container. Then use a balance to accurately weigh an appropriate amount of ammonium acetate solid (if the buffer is made by reacting ammonia with acetic acid, an appropriate amount of ammonia can be measured according to the calculation), and slowly add it to the acetic acid container.

Add and gently stir with a glass rod to fully dissolve the ammonium acetate. If you need to fine-tune the pH, you can add dilute acetic acid or dilute ammonia water dropwise, and monitor with pH until the desired pH is reached. The ammonium acetate buffer prepared by

should be properly stored in a clean and sealed container to prevent it from reacting with foreign objects and causing changes in the composition and affecting its buffering performance. In this way, a suitable ammonium acetate buffer can be obtained.