On the practice of n-hexane-ethyl acetate column chromatography
The separation of substances is multi-ended, and n-hexane-ethyl acetate column chromatography is really one of the wonderful methods.

The basis of this method is derived from the difference in physical properties between the two. For n-hexane, a non-polar solvent is also sparse in its properties, and it has an affinity for non-polar substances. For ethyl acetate, it has a certain polarity and matches with slightly more polar substances. The two are mixed in different proportions, which can be used as the mobile phase of column chromatography, so that the substances on the fixed phase can be eluted one after another due to the difference between adsorption and desorption, and then separated.

When casting, prepare the column first. The column should be clean and uniform, filled with a suitable stationary phase, such as silica gel. Then, the sample to be analyzed is dissolved in an appropriate amount of solvent and carefully added to the top of the column. The slow injection of n-hexane-ethyl acetate mixture is the mobile phase, and the flow rate should be uniform and appropriate, and it should not be diseased or slow. The substances are gradually stratified as the mobile phase descends, due to the different adsorption forces on the stationary phase. Those with small polarity, affinity with n-hexane, exit the column first; those with slightly larger polarity, strong interaction with ethyl acetate, exit the column later. This process requires careful observation and timely collection of each fraction.

n-hexane-ethyl acetate column chromatography is very useful in the fields of organic synthesis and purification of natural products. It can make complex mixtures, organized and in their own place, laying a solid foundation for subsequent research and application. Those who make good use of this method can be used in the chaotic material world to extract pure things, explore their mysteries, and use them for various causes.