On Thin-layer Chromatography of Ethyl Ester and Hexane
It is now the case that Ethyl Acetate and Hexane share in thin-layer chromatography (TLC). Thin-layer chromatography is a method of separating and analyzing substances. In this method, ethyl ester and hexane play a key role.

Ethyl ester, mild in nature, has a certain polarity. For hexane, the polarity is very weak. Mixing the two in different proportions affects the effect of thin-layer chromatography. If the ratio of ethyl ester is high, the polarity of the mixed liquid will increase. During thin-layer chromatography, the polar substances move faster and climb farther on the chromatography plate; if the ratio of hexane is high, the polarity of the mixed liquid will decrease, and the non-polar substances will move more easily.

When operating, it is necessary to carefully adjust the ratio of the two. Try a small amount of test solution first to observe the separation of substances on the chromatography plate. If the separation of substances is poor, or overlaps in one place, or excessive diffusion, the ratio of ethyl ester and hexane should be adjusted in a timely manner. Repeat the test to ensure that different substances are clearly separated on the chromatography plate, and each position is displayed, so that the composition of the substance can be accurately analyzed.

Performing this thin layer chromatography also affects the cleanliness of the instrument and the temperature and humidity of the environment. The instrument is unclean, and impurities are mixed in, which interferes with the chromatography results. Abnormal temperature and humidity also cause the movement speed of the substance to change, making the results deviated. Therefore, when handling, pay attention to all things to achieve accuracy.