On the method of ammonium acetate precipitation of DNA
If you want to obtain pure DNA, there are many methods, and the method of ammonium acetate precipitation is quite delicate.

Nowadays, the science of biology is gradually emerging, and the purification of DNA is crucial for the analysis and testing of genetic materials. Ammonium acetate has a significant effect on DNA precipitation.

When taking a solution containing DNA, slowly add an appropriate amount of ammonium acetate. Ammonium acetate dissociates into ions in the solution. The cation interacts with the charge carried by the impurities in the solution, causing the solubility of the impurities to change, or settle, or combine with other ingredients. In this process, the interaction between DNA molecules also changes due to the influence of ammonium acetate. The repulsive force between DNA molecules is weakened and the attractive force is enhanced, so they aggregate with each other and gradually form a visible precipitate.

In this process, temperature control and the amount of ammonium acetate are all key. If the temperature is too high, DNA may be damaged; improper dosage of ammonium acetate is also difficult to achieve the ideal precipitation effect. Therefore, it is necessary to observe the state of the solution and adjust the conditions in a timely manner.

To form a precipitate, centrifuge to separate the precipitate from the supernatant. Discard the supernatant, leave the precipitate, and then wash the precipitate with an appropriate solvent to remove the residual ammonium acetate and other impurities. After these steps, relatively pure DNA can be obtained.

is a method of precipitating DNA with ammonium acetate. Although it is difficult, if it is carefully studied and used, it is a good method to obtain pure DNA in biological experiments.