Ammonium acetate method for DNA extraction

If you want to obtain pure DNA, there are many methods, and the method of ammonium acetate is particularly important.

Begin, prepare all kinds of utensils, clean and unstained, and put all kinds of agents, ammonium acetate is among them, and accurate weighing must be correct. When the sample arrives, or is taken from a living body, or originates from elsewhere, it must be properly disposed of to ensure its integrity.

Put the sample into a specific container, add an appropriate amount of buffer solution, and disperse it evenly. Then add the solution of ammonium acetate, stir it slowly, and make it fully mixed. In this step, the power of ammonium acetate gradually emerges, interacting with impurities, making DNA unique.

After that, by centrifugation, turn it quickly, the impurities settle down, and the DNA remains in the supernatant. Take the supernatant carefully, so as not to disturb the impurities in the lower layer. Compound with alcohol precipitation, the DNA is then precipitated, and the shape is like a filament, white and pure.

Then use the washing liquid to clear it, remove the remaining impurities, re-centrifuge, collect the DNA, or freeze it, or use it for various experiments.

This ammonium acetate method to extract DNA, although the steps are complicated, it is done in sequence, and with care, pure DNA can be obtained, which can help scientific research.