The method of cellulose acetate film electrophoresis
There is a technique today, which is called cellulose acetate film electrophoresis. The method uses cellulose acetate film as the medium, adds the sample to it, and puts it in an electrophoresis tank to pass an electric current.
Because the cellulose acetate film has a uniform and fine structure, and has a small adsorption effect, it can make the components of the sample swim at different rates under the action of an electric field according to the nature and quantity of the charge they carry, so as to achieve the effect of separation.
Prepare the cellulose acetate film first, cut it to an appropriate size, and infiltrate it with a buffer solution to make it fully absorb moisture. Then take an appropriate amount of sample and carefully add it to the sampling line at one end of the film. Place the film on the bracket of the electrophoresis tank, so that the end of the sample is close to the negative electrode, because the general sample is often negatively charged and swims to the positive electrode. Adjust the voltage and current, and control the time to make the electrophoresis proceed in an orderly manner.
After electrophoresis is completed, take out the film, suitable for staining with dyes, and then rinse to remove excess dye, you can see the separated zone. Depending on the sample, it may be a protein strip or a nucleic acid fragment. The width and depth of this zone can reflect the content of the corresponding components.
The method is simple and fast, with high resolution and a small amount of samples required. It is widely used in biochemical, medical testing and other fields. It is a powerful means to analyze the composition and characteristics of substances.