Cellulose acetate hemoglobin electrophoresis
If you want to know the difference of hemoglobin in detail, cellulose acetate hemoglobin electrophoresis is also the main method. This method uses cellulose acetate as the support medium to distinguish the difference in the charge of different hemoglobin, so that the swimming rate in the electric field is different, and then a clear band is obtained to clarify the type and content of hemoglobin.

Prepare all the required clean cellulose acetate membrane, adjust it to a buffer solution with a suitable pH, take an appropriate amount of blood sample to be tested, and carefully point the sample at a specific place on the membrane. Then place the membrane in an electrophoresis tank to stabilize the current. During this time, each component of hemoglobin slows down to the corresponding electrode according to its charge.

After an appropriate period of electrophoresis, bands at different positions appear on the membrane, just like the stars. Then stained with a specific dye, the bands become more and more clearly identifiable. Looking at its position and color depth, you can know the status of normal or abnormal hemoglobin. This is of great significance in the diagnosis of many blood diseases, such as thalassemia, abnormal hemoglobinopathy, etc., and can point a clear direction for doctors to treat diseases. It is an indispensable skill in medicine.