Cellulose Acetate Electrophoresis Operation Process
Material Preparation
1. Cellulose Acetate Film: Select the appropriate size and pore size of cellulose acetate film to meet the needs of sample separation.
2. Buffer: According to the characteristics of the sample and the purpose of separation, accurately configure the buffer with specific pH value and ionic strength, such as barbiturate buffer.
3. Sample: Proper handling of the sample to be analyzed to ensure that its concentration and purity are suitable for electrophoresis separation. For example, the serum sample needs to be centrifuged to remove impurities.
4. Electrophoresis instrument and electrophoresis tank: Check the performance of the instrument to ensure that the required voltage and current can be provided stably, and the electrophoresis tank is clean and free of impurities. < br Staining solution and eluent: Prepare suitable staining solution, such as amino black 10B staining solution, and the corresponding eluent.

Operation steps
1. ** Film preparation **: Soak the cellulose acetate film in the buffer solution to make it completely wet, then take it out, and gently press it with filter paper to absorb the excess buffer.
2. ** Spotting **: Use a micropipette or spotter to accurately absorb an appropriate amount of sample, and evenly point it on the spotting line of the film. Note that the amount of spotting should not be too much or too little, so as not to affect the separation effect.
3. ** Electrophoresis **: The film after spotting is placed flat on the electrophoresis tank bracket, the spotting end is placed on the negative electrode, and the electrophoresis tank cover is covered. Turn on the power supply, and set the appropriate voltage and current according to the sample characteristics and film specifications. Usually, the voltage is 100 - 200V, and the electrophoresis time is about 30 - 60 minutes.
4. ** Dyeing **: After electrophoresis, carefully remove the film and soak it in the staining solution for several minutes to fully stain the protein and other samples.
5. ** Elution **: Move the stained film to the eluent, gently shake, and wash off the excess staining solution until the background is clear and the strips are distinct.
6. ** RESULTS OBSERVATION AND ANALYSIS **: Observe the position, number and color depth of the strips on the analyzed film with the naked eye or with the help of scanners and other equipment to judge the composition and content of the sample.

Precautions
1. Keep the film moist during operation to avoid deformation of the strips due to drying.
2. Spotting should be rapid and uniform to prevent sample diffusion.
3. Pay close attention to voltage and current changes during electrophoresis to ensure stable electrophoresis conditions.
4. The dyeing and elution time should be strictly controlled so as not to affect the clarity and accuracy of the results.