Cellulose Acetate Electrophoresis
Cellulose acetate electrophoresis is an essential technique for biochemical analysis. The method uses cellulose acetate as the supporting medium. Under the action of an electric field, various charged particles have different swimming rates in the medium according to the nature and amount of charge they carry, as well as the difference in molecular size and shape, so as to achieve separation.

This technique has significant advantages. First, the texture of cellulose acetate is uniform, the pore size is fine and regular, which can make the separation effect excellent and the zone is clear. Second, its electroosmotic effect is weak, and it has little interference with the swimming motion of the separated substance, which can ensure accurate results. Third, the method is convenient to operate and takes less time, making it suitable for rapid analysis of multiple samples.

During the operation, carefully prepare the cellulose acetate film and soak it in the buffer to ensure good electrical conductivity. Then, accurately sample the film, making sure it is uniform and appropriate. Then, place the film in the electrophoresis tank, properly connect the electrodes, set the appropriate voltage and time, and start the electrophoresis process. During this period, pay close attention to the stability of the current and voltage, as well as the change of temperature in the electrophoresis tank.

After the electrophoresis is completed, the film needs to be dyed to make the separated bands clearly visible. Common stains vary according to the nature of the sample. For example, proteins can be stained with amino black. After staining, rinse to remove excess stains, and clear bands can be seen at this time. Through optical density scanning and other means, each component can be quantitatively analyzed, and the content and ratio of each component can be accurately obtained.

Cellulose acetate electrophoresis has key applications in many fields such as biochemistry and clinical diagnosis. In biochemistry, it is often used for the separation and identification of biological macromolecules such as proteins and nucleic acids to help researchers understand the characteristics and structures of biomolecules. In clinical diagnosis, it can be used for serum protein analysis to assist doctors in diagnosing diseases, such as liver disease and kidney disease. Serum protein components are often abnormal. With this technique, it can be keenly detected and provide a strong basis for disease diagnosis and treatment.

In short, cellulose acetate electrophoresis occupies an irreplaceable position in scientific research and medical practice due to its unique advantages, and continues to promote the development and progress of related fields.