"On Carbon and Nitrogen Derived from Pancreatic Soybean Peptone Liquid Medium"

The source of carbon and nitrogen is also the source of nutrients, and the source of carbon and nitrogen is the key element in the pancreatic cheese soybean peptone liquid medium (TSB). Carbon sources, the cornerstone of the building, are the foundation of microbial metabolism and energy supply. There are many kinds of them, sugars, alcohols, organic acids, etc., can be the source of carbon. Among sugars, glucose is often the first choice for microorganisms, because it is easy to ingest and metabolize, and can quickly start the growth engine of microorganisms.

Nitrogen sources should not be ignored, which are necessary for the synthesis of bacterial proteins and nucleic acids. Organic nitrogen sources such as caseone and soybean peptone are rich in a variety of amino acids and peptides, which provide high-quality nitrogen for microorganisms and help them build macromolecules of life. Inorganic nitrogen sources such as ammonium salts and nitrates, although in different forms, are also indispensable nitrogen sources for suitable microorganisms.

Carbon and nitrogen originate from TSB, and the appropriate ratio is crucial. An unbalanced carbon-nitrogen ratio may cause microbial growth retardation or abnormal metabolites. Therefore, by properly adjusting the ratio of carbon and nitrogen sources and accurately adapting to the needs of microorganisms, the ideal culture effect can be obtained. In order to make microorganisms thrive in TSB and produce target products efficiently, it is necessary to deeply study the characteristics, functions and adaptation methods of carbon and nitrogen sources. This is the essence of cultivation and the path to explore the mysteries of microorganisms.