Bacterial growth curve and the investigation of two carbon sources
A certain school palace has the wonders of generating and researching the growth of microorganisms. One day, I wanted to explore the rules for the growth of bacteria in the environment of carbon sources, so I set up an experiment.
Put two utensils, one is fed with methyl carbon source, and the other is raised with ethyl carbon source. All are carefully controlled for temperature, humidity and various conditions, so as to ensure uniformity.
At the beginning, all bacteria are in a period of retardation. In the methyl carbon source, the bacteria gradually adapt to their environment, and the intracellular enzyme system is gradually adjusted to respond to the new source, although slow and stable. The same is true for bacteria in the ethyl carbon source, exploring new frontiers and preparing for proliferation.
Soon, the bacteria in the methyl carbon source enter the period of logarithmic growth. Due to the adaptation of the source, the metabolism is flourishing, and the number increases geometrically. Looking at it, the number of bacteria rises sharply and the vitality is overflowing. At this time, the bacteria in the ethylene carbon source are still adapting, and the growth is slightly slower.
Then, the methylene carbon source is gradually depleted, metabolic waste accumulates, and the growth of bacteria is blocked. In the stable period, the number of bacteria is constant. In the ethylene carbon source, the bacteria are finally adapted, and the growth is logarithmic, and the vitality is gradually strengthened.
After
, the methylene carbon source environment deteriorates, the number of bacteria decreases, and it enters the period of decline. The bacteria in the ethylene carbon source are also gradually exhausted due to nutrient sources, metabolic fatigue, and follow in its footsteps, gradually dying.
Health records data in detail, and plots the growth curve. Looking at it, the growth of bacteria under carbon sources A and E has their own unique paths. A is fast and early and premature, and B is slow and powerful. This verification shows that the carbon source is different, and the growth of bacteria is different. It is the business of studying bacteria and using bacteria for evidence.