On the role of ammonium acetate in DNA extraction
The extraction of human DNA is crucial to biological research. During this period, the role of ammonium acetate is worth studying in detail.
Ammonium acetate has several important functions in the process of DNA extraction. First, it can help the precipitation of impurities such as proteins and polysaccharides. In the capping solution, ammonium acetate can change the solubility of proteins and polysaccharides, causing them to precipitate from the liquid phase, so that they are separated from DNA, making the DNA solution purer and free from impurities.
Second, ammonium acetate also helps significantly in the precipitation process of DNA. It can adjust the ionic strength of the solution, causing DNA molecules to get close to each other, and then precipitate. This process is similar to the return of all rivers to the sea. Under the environment created by ammonium acetate, DNA molecules gradually gather and eventually form a visible precipitate, which is convenient for collection and subsequent processing.
Furthermore, ammonium acetate can stabilize the structure of DNA. During the extraction operation, changes in the external environment may affect the structure of DNA, and the existence of ammonium acetate can maintain a suitable chemical environment, acting as a loyal guardian, protecting the stability of DNA structure, preventing it from damage, and preserving its biological activity and genetic information integrity.
To sum up, ammonium acetate is either a promoter of impurity precipitation in DNA extraction, or a facilitator of DNA precipitation, or a guardian of DNA structure. It has many functions and is indispensable for the success of biological experiments and the advancement of research.