Ammonium acetate RNA precipitation method Detailed discussion of
Fuzumin ammonium acetate RNA precipitation method, when investigating its source, analyzing its rationale, and explaining its use.

The art of autobiochemistry is increasingly profound in the study of nucleic acids. The extraction and purification of RNA is one of the most important tasks of molecular biology. The method of ammonium acetate RNA precipitation, with its unique advantages, occupies a place in all kinds of methods.

Ammonium acetate, with its mild nature, can preserve the integrity of its structure in the precipitation of RNA, preventing it from being damaged by severe conditions. The reason is that the ions dissociated by ammonium acetic acid can interact with the water molecules around RNA, change the ionic strength and polarity of the solution, enhance the interaction between RNA molecules, and then precipitate out of the solution.

To perform this precipitation method, it is necessary to abide by the procedures. First, prepare the sample. The solution containing RNA should be pure, and there should be less heteroproteins and polysaccharides to prevent it from disturbing the effect of precipitation. Next, add an appropriate amount of ammonium acetic acid solution. Depending on the condition of the sample and the amount of RNA, the dose can be precisely controlled. Compounded with alcohol to aid sedimentation, either ethanol or isopropanol can reduce the solubility of RNA and promote its sedimentation. Then, low temperature centrifugation, by the force of centrifugation, separates the precipitation from the supernatant, and RNA is enriched at the bottom of the tube.

Ammonium acetate RNA precipitation method is widely used. In the study of gene expression, pure RNA can be obtained, which is an excellent material for reverse transcription, quantitative PCR and other experiments; in virology, it can help analyze the characteristics of viral RNA; in botany, it can explore the expression and regulation of plant genes.

However, this method is not without defects. The existence of impurities or the precipitation is impure, and the control of temperature and reagent ratio needs to be fine during operation. A little carelessness will damage the quality and quantity of RNA.

Overall, the ammonium acetate RNA precipitation method is a powerful tool for RNA research. Those who use it should be familiar with its principles, adhere to its laws, and promote its strengths while avoiding its weaknesses, so that they can reach a new realm through scientific research.