On the method of ammonium acetate precipitation of DNA
If you want to obtain pure DNA, there are many methods, but the method of ammonium acetate precipitation is quite delicate.

Ammonium acetate has its unique use in the precipitation of DNA. In various situations of DNA extraction, the solution system is complex and there are many impurities. When ammonium acetate is added to it, ammonium acetate can be dissociated, and its ions interact with many components in the solution.

In the extraction of nucleic acid, the solution often contains impurities such as proteins and polysaccharides. The cation of ammonium acetate can interact with the charge on the surface of the protein, causing it to coagulate and then separate from the solution system. For DNA, due to the specific structure and charge characteristics of DNA, ammonium acetate can create a suitable ionic strength environment to promote DNA precipitation.

When operating, follow the procedures. First place the mixed solution containing DNA in a suitable container, slowly add an appropriate amount of ammonium acetate solution, and gently stir it to ensure that the two are evenly mixed. After centrifugation, the precipitation is separated from the supernatant. The force of centrifugation needs to be precisely regulated. If it is too strong, it may damage the structure of DNA, and if it is too weak, it will be difficult to precipitate.

The DNA obtained by precipitation needs to be washed with suitable reagents to remove the residual ammonium acetate and other impurities. After washing, relatively pure DNA can be obtained for subsequent experiments, such as PCR amplification, gene sequencing, etc.

This method of ammonium acetate precipitation of DNA is a good strategy for extracting pure DNA in molecular biology experiments, which can pave a solid foundation for scientific research.