On the Application of Ammonium Acetate in DNA Extraction
The extraction of DNA is the key to biological research, which is related to gene analysis and genetic exploration. Among them, the role of ammonium acetate cannot be underestimated.
Ammonium acetate has its unique effect in the process of DNA extraction. First, it can help the separation of nucleic acid and protein. Cover proteins have different charge states under specific conditions, while ammonium acetate can adjust the ionic strength and pH of the solution, so that the solubility of the protein can be changed, or precipitated, or retained in the solution, and the nucleic acid can be separated from the nucleic acid. In this way, the nucleic acid is purified and subsequent analysis is accurate.
Second, ammonium acetate can promote the precipitation of DNA. DNA is often dissolved in solution, and suitable conditions are required for it to precipitate and collect. The addition of ammonium acetate interacts with the water molecules around DNA, reducing the degree of hydration of DNA and promoting its condensation and precipitation. Like the sand of a river, it aggregates when blocked. DNA can also precipitate and form when it encounters ammonium acetate regulation, making it easy to collect and purify.
Furthermore, it also contributes to the structural stability of DNA. During the extraction operation, external factors are changeable, and DNA is vulnerable to damage. Ammonium acetate can create a relatively mild environment, maintain the stability of the DNA double helix structure, and keep its genetic information intact. Like a sturdy castle, it protects DNA from mutation and damage during the complicated process of extraction.
In short, ammonium acetate is an indispensable element for DNA extraction, or to isolate protein nucleic acids, or to promote DNA precipitation, or to protect its structural stability. It is of great significance in the process of biological research.