On the application of ammonium salts in high performance liquid phase
Ammonium salts are of great significance in the field of high performance liquid chromatography (HPLC). Today, ammonium acetate (Ammonium Acetate For HPLC) is the core of analysis, and its characteristics and effectiveness are studied.

Ammonium acetate, in HPLC, is often the buffer of choice. It can stabilize the pH value of the mobile phase and maintain the stability of the chromatographic environment. The quality of HPLC separation is sensitive to environmental pH. If the pH fluctuates, the separation effect and the shape of the peak are disturbed by it. Ammonium acetate, by virtue of its own chemical properties, can regulate the concentration of protons in a specific pH range, making the pH of the mobile phase constant, so that the components to be precipitated can be separated in sequence and clearly.

And its solubility is quite good, it can be dissolved in common solvents, such as water and some organic solvents. This characteristic makes it convenient and easy to prepare the mobile phase, and it can be evenly dispersed, without affecting the analysis process of HPLC due to difficulty in dissolving.

Furthermore, ammonium acetate has little background interference in the detection of HPLC. It does not interfere with common detectors, such as ultraviolet detectors, fluorescent detectors, etc., to generate strong interference signals. Therefore, the accuracy of the test results can be guaranteed, and the response of the peak is real, so that the analyst can present accurate data and help him analyze the composition and content of the sample.

Looking at the whole process of HPLC, from the matching of the mobile phase to the analysis of the sample, and then to the judgment of the results, Ammonium acetate (Ammonium Acetate For HPLC) is a key factor, which is essential for the separation and analysis of the industry.